PH EFFECT ON HUMAN ERYTHROCYTE SHAPE

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PH EFFECT ON HUMAN ERYTHROCYTE SHAPE

ABSTRACT

The effect of pH on shape of intact erythrocyte was studied by suspending erythrocyte in phosphate buffer saline of pH outside the range 8.0 to 4.0. These were found to induced instantly two typical types of shape changes of human erythrocyte when added into the erythrocyte suspension. Generally, as the pH of the suspending media decreased toward acidity (pH 3.0 – 1.0) cup shape calls (invagmation) were formed. Subsequently, this was accomplished by cell lyrics. On the other hand, increasing pH of the suspending media toward – alkalinity (pH 8.0 – 12.0) induced crenation (externalization) on the erythrocyte membrane. Accompanying this type of shape change were a progressive increased in cell lyrics which began as pH of the media increased to pH 11.0. Ghost cells also formed at this pH

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