BIOREMEDIATION OF HEXAVALENT CHROMIUM CONTAMINATION IN WATER BY PSEUDOMONAS CORRUGATA

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BIOREMEDIATION OF HEXAVALENT CHROMIUM CONTAMINATION IN WATER BY PSEUDOMONAS CORRUGATA

Abstract

Hexavalent chromium (Cr(VI)) is a highly toxic and carcinogenic pollutant commonly found in industrial wastewater, posing significant environmental and public health risks. This study investigates the potential of Pseudomonas corrugata, a versatile bacterium, in the bioremediation of Cr(VI) contamination in water. The research explores the efficiency of P. corrugata in reducing Cr(VI) to the less toxic trivalent chromium (Cr(III)) under varying environmental conditions, including pH, temperature, and initial Cr(VI) concentration.

Laboratory experiments were conducted to assess the reduction capacity of P. corrugata, where the bacterium was exposed to synthetic Cr(VI)-contaminated water samples. The reduction process was monitored over time using spectrophotometric analysis, and the results were analyzed to determine the optimum conditions for maximum bioremediation efficiency.

Key findings indicate that P. corrugata effectively reduced Cr(VI) levels by over 90% under optimal conditions, with the highest reduction rates observed at a pH of 7 and a temperature of 30°C. The study also found that the bacterium’s Cr(VI) reduction capability was concentration-dependent, with higher initial Cr(VI) concentrations leading to slower reduction rates. Additionally, the formation of biofilms by P. corrugata was observed to enhance the reduction process, suggesting that biofilm-mediated bioremediation could be a promising approach for treating Cr(VI)-contaminated water.

This research demonstrates the potential of Pseudomonas corrugata as an effective biological agent for the remediation of Cr(VI) contamination in water. The findings contribute to the development of sustainable and eco-friendly bioremediation strategies for heavy metal pollution, offering a viable alternative to conventional chemical and physical treatment methods.

Keywords: Bioremediation, Hexavalent Chromium, Pseudomonas corrugata, Water Contamination, Biofilm, Environmental Remediation.

Chapter One: Introduction

1.1 Background of the Study

Hexavalent chromium (Cr(VI)) is a widespread environmental pollutant, primarily generated through industrial activities such as leather tanning, electroplating, and the production of dyes and pigments. Due to its high solubility in water, Cr(VI) often contaminates groundwater and surface water, posing severe risks to ecosystems and human health. Exposure to Cr(VI) is known to cause various adverse effects, including carcinogenic, mutagenic, and teratogenic impacts. Consequently, the remediation of Cr(VI)-contaminated water has become a critical environmental challenge.

Traditional methods for removing Cr(VI) from water, such as chemical precipitation, ion exchange, and adsorption, often involve high costs and can produce secondary pollution. As a result, there is growing interest in developing sustainable and eco-friendly alternatives, such as bioremediation. Bioremediation leverages the natural metabolic processes of microorganisms to detoxify contaminants, offering a cost-effective and environmentally benign approach to pollution management.

Pseudomonas corrugata, a gram-negative bacterium known for its metabolic versatility and environmental resilience, has shown potential in the bioremediation of various heavy metals, including chromium. This bacterium can reduce Cr(VI) to the less toxic trivalent chromium (Cr(III)), which is insoluble in water and less bioavailable, thereby mitigating the environmental and health risks associated with Cr(VI) contamination.

1.2 Statement of the Problem

Despite the recognized potential of bioremediation, the application of Pseudomonas corrugata in the remediation of Cr(VI)-contaminated water remains underexplored. Current knowledge about the efficiency, mechanisms, and optimal conditions for Cr(VI) reduction by P. corrugata is limited. Additionally, while the formation of biofilms by P. corrugata has been observed to enhance its bioremediation capabilities, the specific role of biofilms in Cr(VI) reduction requires further investigation.

Given the toxic nature of Cr(VI) and the limitations of conventional treatment methods, it is imperative to explore and optimize bioremediation strategies using Pseudomonas corrugata. This study addresses the need for a deeper understanding of the bioremediation potential of P. corrugata and seeks to identify the conditions under which this bacterium can most effectively reduce Cr(VI) in contaminated water.

1.3 Objectives of the Study

The primary objectives of this study are to:

Evaluate the Cr(VI) Reduction Efficiency of Pseudomonas corrugata: Assess the ability of P. corrugata to reduce Cr(VI) to Cr(III) in water under various environmental conditions.

Investigate the Influence of Environmental Factors: Determine the impact of pH, temperature, and initial Cr(VI) concentration on the reduction process.

Examine the Role of Biofilm Formation: Explore the contribution of biofilm formation by P. corrugata to the bioremediation process and its potential to enhance Cr(VI) reduction.

Optimize Bioremediation Conditions: Identify the optimal conditions for maximizing the efficiency of Cr(VI) reduction by P. corrugata.

1.4 Research Questions

This study seeks to answer the following research questions:

How effectively does Pseudomonas corrugata reduce Cr(VI) to Cr(III) in contaminated water?

What are the optimal environmental conditions (pH, temperature, Cr(VI) concentration) for the bioremediation of Cr(VI) by Pseudomonas corrugata?

How does biofilm formation by P. corrugata influence the reduction of Cr(VI)?

What are the implications of Pseudomonas corrugata bioremediation for environmental management and water treatment?

1.5 Significance of the Study

This study is significant for several reasons:

Advancement of Bioremediation Science: By exploring the bioremediation potential of Pseudomonas corrugata, this study contributes to the broader field of environmental microbiology and the development of sustainable remediation technologies.

Environmental and Public Health Benefits: Effective bioremediation of Cr(VI) contamination can mitigate the environmental and health risks associated with this toxic pollutant, contributing to cleaner water resources.

Practical Applications: The findings of this study can inform the design and implementation of bioremediation strategies in industrial wastewater treatment and environmental management practices.

Policy Implications: Insights gained from this research could support the development of policies and regulations promoting the use of bioremediation for heavy metal contamination.

1.6 Scope

This study focuses on the bioremediation of Cr(VI) contamination in water using Pseudomonas corrugata. The research examines the bacterium’s reduction efficiency under various environmental conditions and investigates the role of biofilm formation in enhancing the bioremediation process.

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